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Fertility Glossary

2PN (two pronuclei)

The visible sign, on day 1, that an egg has fertilised normally.

Eggs, sperm & embryos

2PN describes the appearance of two small circles inside the egg on the morning after insemination, each containing genetic material from one parent. Seeing 2PN is how laboratories confirm normal fertilisation before the embryo starts dividing.

Also known as: 2PN, pronuclei, PN check

Sources

  • ESHRE/ICMART glossary and ESHRE laboratory guidance

Last reviewed: 8 September 2026. Written by the Antega Editorial Team. General information only β€” always follow the advice of your own clinic, doctor or pharmacist.

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